Krittika Mittal
Postdoctoral Fellow, McGill University Department of Natural Resource Sciences Faculty of Agricultural and Environmental Sciences [email protected]; +1 514 618-9269 |
SUMMARY
I am broadly trained ecotoxicologist with 8 years’ research experience in in vivo and in vitro toxicity testing
I am broadly trained ecotoxicologist with 8 years’ research experience in in vivo and in vitro toxicity testing
- Skilled bench scientist with 6 years’ experience in molecular biology and biochemistry
- Extensive experience in developing, optimizing and troubleshooting techniques
- Excellent communication skills honed through providing training and demonstrations to students and technicians
EDUCATIONAL BACKGROUND
PhD McGill University, Macdonald Campus (2014-2018)
Department of Natural Resource Sciences
Faculty of Agricultural and Environmental Sciences
MS University of Michigan, Ann Arbor, Michigan (2009-2011)
Department of Biomedical Engineering
Concentration: Biotechnology
BE People's Education Society Institute of Technology (PESIT) (2005-2009)
Visvesvaraya Technological University, Bangalore
Major: Biotechnology
PhD McGill University, Macdonald Campus (2014-2018)
Department of Natural Resource Sciences
Faculty of Agricultural and Environmental Sciences
MS University of Michigan, Ann Arbor, Michigan (2009-2011)
Department of Biomedical Engineering
Concentration: Biotechnology
BE People's Education Society Institute of Technology (PESIT) (2005-2009)
Visvesvaraya Technological University, Bangalore
Major: Biotechnology
SKILLS
- Molecular biology: RNA/DNA isolation and quantification, gel electrophoresis, reverse transcription, qPCR (single target, multiplex, RT2 Profiler PCR arrays), RNA-seq, pyrosequencing
- Instruments: QIAgility, QIAcube, QIAxpert, QIAxcel, PyroMark Q24, Biorad CFX Touch 96/384, PerkinElmer Wallac 1450 Microbeta Trilux, Bruker S2 Picofox, Milestone DMA-80
- Miscellaneous: in vivo and in ovo studies, cell culture, dissections, protein quantification, ELISA, high throughput cell-free assays, metals analyses
- Computer: proficiency with MS Office suite, and statistical software (XL STAT and JMP),
- Communication: provided training to lab personnel; poster and platform presentations at national and international conferences; co-authored numerous manuscripts and a book chapter
àRESEARCH AND PROFESSIONAL EXPERIENCE
Postdoctoral Fellow, McGill University, Ste-Anne-de-Bellevue, Quebec, Canada 2018-present
*Supervisor: Dr. Nil Basu
Applying alternative toxicity testing methods to real world situations with a focus on how toxicity of pristine nanoparticles and nanoparticles released from painted surfaces differ on outcomes such as uptake, gene expression, enzyme activity, and cell viability.
PhD Candidate, McGill University, Ste-Anne-de-Bellevue, Quebec, Canada 2014-2018
*Supervisor: Dr. Nil Basu
Research Associate, University of Michigan, Ann Arbor, Michigan, USA 2011-2013
*Supervisor: Dr. Nil Basu.
Intern, GI Molecular Motors Lab, University of Michigan, Ann Arbor, USA 2010
Suprevisor: Prof. Khalil Bitar
Intern, Inbiopro Solutions Pvt. Ltd., Bangalore, India 2009
Supervisor: Hardik Valera
Studying the effects of different media components on the growth of rE.coli, recombinant protein expression and lysis efficiency
Postdoctoral Fellow, McGill University, Ste-Anne-de-Bellevue, Quebec, Canada 2018-present
*Supervisor: Dr. Nil Basu
Applying alternative toxicity testing methods to real world situations with a focus on how toxicity of pristine nanoparticles and nanoparticles released from painted surfaces differ on outcomes such as uptake, gene expression, enzyme activity, and cell viability.
PhD Candidate, McGill University, Ste-Anne-de-Bellevue, Quebec, Canada 2014-2018
*Supervisor: Dr. Nil Basu
- Developed alternative methods of studying disruption in endocrine function and xenobiotic metabolism in chicken embryonic liver using slice culture
- Examined the evolving field of ecotoxicology and assessing whether alternative toxicity testing methods can be more resource-efficient than traditional animal-based methods
- Planned and executed an in vivo study to examine toxicogenomic and biochemical effects of dietary 17β-trenbolone in Japanese quail
- Investigated the effects of in ovo and dietary 17β-trenbolone exposure on the Japanese quail hepatic transcriptome
Research Associate, University of Michigan, Ann Arbor, Michigan, USA 2011-2013
*Supervisor: Dr. Nil Basu.
- Identified and optimized high throughput cell-free screening assays to test neurochemical effects of chemicals in multiple species
- Demonstrated exemplary laboratory skills through toxicological studies examining the effects of mercury and organophosphate flame-retardants and looking at epigenetic changes (methylation) in DNA from multiple species
Intern, GI Molecular Motors Lab, University of Michigan, Ann Arbor, USA 2010
Suprevisor: Prof. Khalil Bitar
- Studied the genetic differences between circular, longitudinal and internal anal sphincter cells of the gastro-intestinal tract.
- Performed force transducer experiments to test bioengineered gastro-intestinal tract smooth muscle implants and tissues from rats, mice and rabbits.
Intern, Inbiopro Solutions Pvt. Ltd., Bangalore, India 2009
Supervisor: Hardik Valera
Studying the effects of different media components on the growth of rE.coli, recombinant protein expression and lysis efficiency
PUBLICATIONS
Peer-reviewed Manuscripts
Manuscripts in Preparation
Book Chapter
Peer-reviewed Manuscripts
- Mittal K., Crump D., & Basu N. (2019). A Comparative Study of Three Alternative Avian Toxicity Testing Methods: Effects on Hepatic Gene Expression in the Chicken Embryo. Environmental Toxicology and Chemistry. DOI: 10.1002/etc.4555.
- Arini A., Mittal K., Dornbos P., Head J., Rutkiewicz J., & Basu N. (2017). A cell-free testing platform to screen chemicals of potential neurotoxic concern across twenty vertebrate species. Environmental Toxicology and Chemistry, 36(11), 3081-3090.
- Bradley M., Rutkiewicz J., Mittal K., Fernie K., & Basu N. (2015). In ovo exposure to organophosphorous flame retardants: survival, development, neurochemical, and behavioural changes in white leghorn chickens. Neurotoxicology and Teratology, 52, 228-235.
- Head, J. A., Mittal, K., & Basu, N. (2014). Application of the LUminometric Methylation Assay to ecological species: tissue quality requirements and a survey of DNA methylation levels in animals. Molecular Ecology Resources, 14(5), 943-952.
- Rutkiewicz, J., Bradley, M., Mittal, K., & Basu, N. (2013). Methylmercury egg injections: Part 2—pathology, neurochemistry, and behaviour in the avian embryo and hatchling. Ecotoxicology and Environmental Safety, 93, 77-86.
- Miyasaka, E. A., Raghavan, S., Gilmont, R. R., Mittal, K., Somara, S., Bitar, K. N., & Teitelbaum, D. H. (2011). In vivo growth of a bioengineered internal anal sphincter: comparison of growth factors for optimization of growth and survival. Pediatric Surgery International, 27(2), 137-143.
Manuscripts in Preparation
- Mittal K., Crump D., Head J., Hecker M., Hogan N., Xia J., Hickey G., Maguire S., & Basu N. Can Alternative Toxicity Testing Methods be more Efficient than Traditional Methods? In prep, expected submission September 2019.
- Mittal K., Henry P.F.P., Cornman R.S., Sprague D.T., & Basu N., Karouna-Renier N.K. Sex- and Developmental Stage-related Differences in the Hepatic Transcriptome of Japanese quail (Coturnix japonica) Exposed to 17β-Trenbolone. In prep, expected submission October 2019.
- Mittal K., Henry P.F.P., Armstrong B., Murphy C., Basu N., & Karouna-Renier N.K. Assessing Short-Term Effects of 17β-Trenbolone on the Japanese Quail Hypothalamic-Pituitary-Gonadal-Liver Axis. In prep, expected submission November 2019.
Book Chapter
- Arini A., Mittal K., & Basu N. (2018). Cell-free assays in environmental toxicology. A Systems Biology Approach to Advancing Adverse Outcome Pathways for Risk Assessment. Springer, Cham, pp. 31-41.
PRESENTATIONS
Platform Presentations
Selected Posters
Platform Presentations
- Mittal K, Crump D, Head J, Hecker M, Hogan N, Xia J, Hickey G, Maguire S, & Basu N. Can Alternative Toxicity Testing Methods Be More Efficient Than Traditional Methods? Society of Environmental Toxicology and Chemistry, Sacramento, 2018.
- Mittal K, Crump D, & Basu N. Comparing effect of chemicals on gene expression using chicken hepatocyte, liver slice culture and in ovo injected embryonic liver. Society of Environmental Toxicology and Chemistry, Minneapolis, 2017.
- Mittal K, Crump D, & Basu N. Comparing effect of chemicals on gene expression using chicken hepatocyte, liver slice culture and in ovo injected embryonic liver. Society of Environmental Toxicology and Chemistry, Minneapolis, 2017.
- Mittal K, Henry P.F.P, Cornman R.S, Sprague D.T, Basu N, & Karouna-Renier N.K. Differential hepatic gene expression analysis of in ovo and dietary 17β-trenbolone effects on Japanese quail using RNA-Seq. Society of Environmental Toxicology and Chemistry, Orlando, 2016.
- Mittal K, Johnston T, & Basu N. In vitro tissue culture model to study neuroendocrine disruption in embryonic chicken. International Neurotoxicology Association - 15, Montreal 2015.
Selected Posters
- Mittal K, Rahim A.A, Ghoshal S, George S, & Basu N. Studying elemental release of engineered nanoparticles used in outdoor painted surfaces with a focus on exposure and toxicity in aquatic models. Society of Environmental Toxicology and Chemistry, Sacramento, 2018.
- Mittal K, Bidinosti L, & Basu N. Development of an in vitro slice culture model for toxicological evaluation of model test compounds in chicken embryo liver. Society of Environmental Toxicology and Chemistry, Orlando, 2016.
- Mittal K, Crump D, Head J, Hecker M, Hogan N, Xia J, Hickey G, Maguire S, & Basu N. Is alternative toxicity testing faster, cheaper and more efficient than traditional methods? Society of Environmental Toxicology and Chemistry, Orlando, 2016.
- Mittal K, Henry P.F.P., Armstrong B, Bidinosti L, Murphy C, Basu N, & Karouna-Renier N.K. Assessing toxicogenomic Effects of 17β-Trenbolone on the Japanese Quail Hypothalamic- Pituitary-Gonadal-Liver Axis. Society of Environmental Toxicology and Chemistry, Orlando, 2016.
- Mittal, K, Johnston T, & Basu N. Development of a tissue co-culture model of the chicken hypothalamus-pituitary-gonadal-liver (HPG-L) axis for in vitro toxicological evaluation. Society of Environmental Toxicology and Chemistry, Vancouver, 2014.
HONOURS AND AWARDS
2018 McGill University International Tuition Bursary 1,500 CAD
2014-2017 McGill University Graduate Excellence Award 70,000 CAD
2017 McGill Graduate Mobility Award 2,100 CAD
2017 McGill University Graduate Research Enhancement and Travel Award 300 CAD
2016 SETAC Student Travel Award 600 USD
2016 SETAC Student Exchange Opportunity Award 600 USD
2015 McGill University Quebec Tuition Bursary 3000 CAD
2015 McGill University Graduate Mobility Award 640 CAD
2014-2015 McGill University Differential Fee Waiver 11,500 CAD
2014 McGill University Graduate Research Enhancement and Travel Award 300 CAD
2005-2009 PES Institute of Technology, First Class with Distinction NA
2018 McGill University International Tuition Bursary 1,500 CAD
2014-2017 McGill University Graduate Excellence Award 70,000 CAD
2017 McGill Graduate Mobility Award 2,100 CAD
2017 McGill University Graduate Research Enhancement and Travel Award 300 CAD
2016 SETAC Student Travel Award 600 USD
2016 SETAC Student Exchange Opportunity Award 600 USD
2015 McGill University Quebec Tuition Bursary 3000 CAD
2015 McGill University Graduate Mobility Award 640 CAD
2014-2015 McGill University Differential Fee Waiver 11,500 CAD
2014 McGill University Graduate Research Enhancement and Travel Award 300 CAD
2005-2009 PES Institute of Technology, First Class with Distinction NA
WHEN I AM NOT IN THE LAB
I like to read, listen to music, run, play squash, hike, camp, travel, meet new people...
I like to read, listen to music, run, play squash, hike, camp, travel, meet new people...